Thermally Responsive ppNIPAM Thin Films
Protein and Cell Arrays on Thermally Responsive Polymers
Yanbing Wang, Yael Hanein, Ashutosh Shastry, Denice Denton, Karl F. Böhringer
Xuanhong Chen, Buddy Ratner (Bioengineering)
Programmable surface chemistry has been achieved by depositing a temperature
sensitive polymer onto arrays of micro-fabricated metallic heaters. Activating
a single heater causes a localized change in the device surface chemistry
from non-fouling to fouling in an aqueous environment. Two types of proteins
and two types of cells were used to demonstrate localized immobilization
of proteins and cells on such surfaces. These experiments show, for the
first time, selective cell attachments on thermally responsive polymer controlled
by a micro heater array. It suggests a new approach to realize proteomic
chips and cell chips.
|Figure 1: . Schematic description
of ppNIPAM devices. (a) Devices consist of micro-heaters on a glass
slide coated with ppNIPAM. (b) Active heater (dark) turns the surface
fouling. Selective protein/cell adsorption occurs exclusively on heated
areas. Temperature sensitive paint was used to characterize the temperature
profile. (c) A second adsorption step with different protein/cell
solution on a different heater.
||Figure 2: Two steps of cell attachments
on the top and bottom heater, respectively. (a) Bright field mode
and (b) Fluorescence mode.
- Xuanhong Cheng, Yanbing Wang, Yael Hanein, Karl F. Böhringer,
Buddy D. Ratner, "Novel cell patterning using microheater-controlled
thermoresponsive plasma films." Journal of Bio-medical Materials
Research Part A, 70A(2):159-168, 1 August 2004. Won the Student
Research Award in the Doctoral Degree Candidate Category, 7th Biomaterials
World Congress, Sydney, Australia, May 16-21, 2004. Paper.
- Yanbing Wang, Xuanhong Cheng, Yael Hanein, Ashutosh Shastry, Denice
D. Denton, Buddy D. Ratner, Karl F. Böhringer, "Selective
Attachment of Multiple Cell Types on Thermally Responsive Polymer."
The 12th International Conference
on Solid-State Sensors and Actuators (Transducers'03), Boston,
MA, June 6-12, 2003. Paper.
- Yanbing Wang, Xuanhong Cheng, Yael Hanein, Buddy Ratner, Karl F. Böhringer,
"Protein Patterning with Programmable Surface Chemistry Chips." Sixth
International Conference on Miniaturized Chemical and Biochemical Analysis
Systems (Micro TAS 2002), vol.1 pp. 482-484, Nara, Japan, November
3-7, 2002. Paper.
A complete list of our publications
(many of them available online) can be found here.
- NIH National Human Genome Research Institute, Centers of Excellence
in Genomic Science, Grant Number 1 P50 HG002360-01, CEGSTech: Integrated
- National Science Foundation, SGER grant ECS-0223598.
© Karl F. Böhringer, Department of Electrical Engineering, Box
352500, Seattle, WA 98195-2500, USA